Regulatory

Part:BBa_K1537015:Design

Designed by: Jie Li   Group: iGEM14_UESTC-China   (2014-08-18)


35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

We have to remove some restriction endonuclease cutting sites by changing a few bases.


Source

The 35S promoter has a large area of application in genetically modified plants.The translation initiation optimized sequence for dicot is from the paper "translation initiation optimized sequence for dicot"(Nucleic Acids Research Volume 36, Issue 3Pp. 861-871 So Nakagawa1, Yoshihito Niimura2, Takashi Gojobori3,4, Hiroshi Tanaka1,2,* and Kin-ichiro Miura5).The mass translation enhancer is from "Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants"（Samir V. Sawant, Kanti Kiran, Pradhyumna K. Singh, and Rakesh Tuli*）

References